Human FVIII procoagulant activity (FVIIIC) is carried by a polypeptide non-covalently bound to a large carrier molecule (unit molecular weight 250kD) known as the Von Willebrand factor (VWF).
FVIII/VWF exists in plasma as a series of polymers with molecular weights >1100 kD.
FVIIIC is probably formed in the liver, is unstable on storage but sufficiently stable in fresh frozen plasma as cryoprecipitate or FVIII concentrate.
VWF is synthesized in megakaryocytes and vascular endothelial cells; it is also present in the alpha-granules and membranes of platelets, binding to specific sites on the activated platelet after its release.
It is responsible for platelet adhesion to the vascular subendothelium.
FVIIIC, but not VWF is completely consumed during coagulation ad is absent from serum.
Polyclonal antisera are raised against FVIII/VWF purified from plasma.
Applications:Suitable for use in Immunoelectrophoresis, Bi-dimensional Electrophoresis and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml sterile dH2O.
Specificity：Recognizes human Factor VIII. Does not react with other varients of human plasma proteins.
