This antibody recognizes a protein of 54kD, which is identified as AMACR, also known as p504S.
It is an enzyme that is involved in bile acid biosynthesis and b-oxidation of branched-chain fatty acids.
AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma.
The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction.
AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia.
A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
Applications: Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100-1:200 for 2 hours at RTImmunohistochemistry: formalin-fixed: 1:50-1:100 for 30-60 minutes at RT(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutesOptimal dilutions to be determined by the researcher.
Positive Control:HEK cells or Prostate Adenocarcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity column.
Form：Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168645-AF.
Specificity：Recognizes human AMACR (p504S).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide from human AMACR protein. Cellular Localization: Cytoplasmic
