The genus Salmonella is a member of the family Enterobacteriaceae.
The genus is composed of Gram negative bacilli that are facultative and flagellated (motile).
Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Endotoxins evoke fever and can activate complement, kinin and clotting factors.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：250ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes Salmonella. Polyvalent for Salmonella O and H antigens. Species Crossreactivity: mouse. Does react with related Enterobacteriaceae.
Isotype：IgG
Calc Applications Abbrev：E IHC
Immunogen：Mixture of S. enteriditis, S. typhimurium and S. heidelburg. Cellular Localization: Whole cell
