Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM).
It is a highly specialized bio-membrane made by terminally differentiated urothelial cells.
The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells.
UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection .
UPK3A has been shown as a helpful marker for the detection of bladder cancer.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlFlow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:HEK2 93 cells or bladder carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS. Also available with BSA and azide, see 138601.
Specificity：Recognizes human UPK3A.
Isotype：IgG
Calc Applications Abbrev：FC IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa260-279 (GASESSYTSVNRGPPLDRAE) from human UPK3A.
