TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM).
An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors.
TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlWestern Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:Placenta or breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS. Also available with BSA and azide, see 138599.
Specificity：Recognizes human TIMP3. Species Crossreactivity: mouse, rat, bovine, canine and equine.
Isotype：IgG
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Bo Ca Eq Hu Mo Rt
Immunogen：Peptide corresponding to aa175-211 from TIMP3. Species Sequence Homology: mouse, rat
