Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.
There are two closely related napsins, napsin A and napsin B.
Napsin A is expressed as a single chain protein.
Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen.
Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung.
The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlWestern Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:Lung adenocarcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS. Also available with BSA and azide, see 138575.
Specificity：Recognizes human Napsin-A.
Isotype：IgG
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide RFDPKASSSFQANGTKFAIQYGT corresponding to human Napsin-A.
