CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha).
A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a.
CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4.
It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-.
Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed.
CD8 is also found in littoral cell angioma of the spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateFlow Cytometry: 1-2ug/10e6 cellsWestern Blot: 1-2ug/mlImmunohistochemistry (Frozen & Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTOptimal dilutions to be determined by the researcher.
Positive Control:HuT78 or hPBL.
Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS. Also available with BSA and azide, see 138530.
Specificity：Recognizes human CD8a.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa200-250 from human CD8a.
