The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells.
One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness.
The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter.
Several other growth related promoters are also regulated by ZBP-89.
ZBP-89 is one of a family of related transcriptional regulators.
It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300.
Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53.
ZBP-89 also induces apoptosis, but this process occurs independent of p53.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:30,000Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human ZBP-89.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ZBP-89 expressed in E.coli.
