The WNT gene family consists of structurally related genes which encode secreted signaling proteins.
These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.
Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family.
It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level.
Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum.
This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature.
However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome.
Wnt1 is secreted as an extracellular matrix protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:25,000Western Blot: 1:1500-1:6000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Wnt1. Species Crossreactivity: mouse, rat, bovine, canine, macaque and opossum.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mc Mo Rt
Immunogen：Synthetic peptide corresponding to human Wnt1.
