Epitope tags are short peptide sequences that are easily recognized by tag-specific.
Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.
Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows epitope tags to serve as universal detection reagents for any tag-containing protein produced by recombinant means.
This means that epitope tags are a useful alternative to generating specifics to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The epitope tag is usually functional in a variety of dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease free), 0.01% sodium azide. Reconstitute with sterile dH2O. Labeled with Biotin.
Specificity：Recognizes VSV-G Epitope Tag.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
