VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells.
VEGF forms homodimers and exists in four different isoforms.
Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended.
VEGF shares homologies of about 21% and 24% respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains.
The cysteine knot motif is a common feature of this domain.
The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo and heterodimers of the PDGF chains.
This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.
VEGF-A also has been shown to have effects on a number of other cell types (e.g.
stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ).
VEGF-A is also a vasodilator; it increases microvascular permeability, and was originally referred to as vascular permeability factor.
Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:333Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes bovine VEGF. Species sequence homology: porcine, sheep, and macaque (100%), human, equine, canine, feline, or guinea pig (95%) rat, and mouse (93%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to 164aa from bovine VEGF-A, expressed in yeast.
