Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as tumor angiogenesis Factor.
Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types.
In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis.
VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.
Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced.
Rat and bovine VEGF are one aa shorter than the human factor, and the bovine and human sequences show a homology of 95 percent.
In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26aa.
Glycosylation is not required for efficient secretion of VEGF.
VEGF121 is acidic, freely secreted, and widely expressed.
This isoform is produced by alternative promoter usage and alternative initiation.
It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF.
The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes human VEGF.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human VEGF expressed in E.coli.
