Epitope tags are short peptide sequences that are easily recognized by tag-specific.
Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows epitope tag to serve as universal detection reagents for any tag containing protein produced by recombinant means.
This means that epitope tag are a useful alternative to generating specific to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The epitope tag is usually functional in a variety of dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:60,000Western Blot: 1:5000-1:10,000Immunohistochemistry: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Affinity Purified
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes V5 Epitope Tag.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Synthetic peptide corresponding to aa95-108 of the V protein conjugated to KLH using maleimide.
