UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin- conjugating enzyme family.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:2000, expect a band ~21kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human UBC12. Species Crossreactivity: chimpanzee, frog, mouse, rat and canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Fr Hu Mo Rt
Immunogen：Synthetic peptide corresponding a C-terminal region near aa169-180 from human UBC12.
