Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape.
Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin).
A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter.
Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase.
Detyrosinated A-tubulin is referred to as G-tubulin.
Another post-translational modification of detyrosinated aAtubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
This makes an excellent loading control.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.. Labeled with Biotin.
Specificity：Recognizes human alpha tubulin. Species Crossreactivity: mouse, rat, chicken, bovine, goat, and sheep.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Ch Gt Hu Mo Rt Sh
Immunogen：Synthetic peptide corresponding to aa425-451 at the C-Terminal of human alpha tubulin.
