Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape.
Tubulin itself is a globular protein consisting of two polypeptides (a and b-tubulin).
A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter.
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase.
Detyrosinated alpha tubulin is referred to as Glu-tubulin.
Another post-translational modification of detyrosinated A-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
This makes an excellent loading control.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% gentamicin s]ulfate, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human A-tubulin. Species Crossreactivity: mouse, rat, chicken, bovine, goat and sheep.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ch Gt Hu Mo Rt Sh
Immunogen：Synthetic peptide corresponding to aa427-441 from human A-tubulin.
