Tubulin Loading Control recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape.
Tubulin itself is a globular protein consisting of two polypeptides (a and b-tubulin).
A and b-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter.
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase.
Detyrosinated a-tubulin is referred to as Glu-tubulin.
Another post-translational modification of detyrosinated a-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
This makes an excellent loading control.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2000ELISA: 1:5000Western Blot: 1:500-1:3000Immunohistochemistry: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human A-tubulin. Species Crossreactivity: mouse, rat, chicken and bovine.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Ch Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa427-441 from human A-tubulin.
