Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses.
These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors.
TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus.
Activation of TLR7 by VSV results in stimulation of the immune response including IFN? secretion, suggesting the importance of TLR7 in virus recognition.
TLR7 is ideal for investigators invleved in kinases, phosphatase, cytokines, growth factors and transcription factor research.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1-2ug/ml, a ~121kD band is expected in the appropriate cell lysate or extractImmunohistochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography from serum.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes mouse TLR7. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to an internal region of mouse TLR7.
