Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage.
Tousled-like kinases, first described in arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.
Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase.
This is cell cycle checkpoint and ATM-pathway dependent.
Tlk-1 heterodimerizes with Tlk-2.
Multiple isoforms of the protein have been detected.
Mature Tlk-1 is reported to have an apparent molecular weight of~86kD,~89kD and~64kD corresponding to isoforms 1, 2 and 3 respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:200-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human TIk1. Species Crossreactivity: chimpanzee, orangutan and canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu
Immunogen：Synthetic peptide corresponding to aa737-751 from human Tlk1.
