Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses.
These proteins act through adaptor molecules such as TIRAP (Toll-interleukin 1 receptor domain-containing adaptor protein) and MyD88 to activate various kinases and transcription factors.
In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 or TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling.
Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kB and MAP kinase activation, suggesting that TIRAP is also involved in TLR4 signaling.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1:500-1:3000, a ~34kD band is expectedImmunohistochemistry: 2ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse TIRAP. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the C-terminal of mouse TIRAP.
