Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine.
THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone.
Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone.
THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes.
THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants.
This recognizes THRA isoform 1.
Isoform 1 has a distinct C-terminus compared to isoform 2.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:300,000Western Blot: 1:500-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human THRA.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human THRA isoform 1 at N-terminal.
