TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27kD catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV).
It is widely used for cleaving fusion proteins because of its sequence specificity.
It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S).
Cleavage occurs between Q and G or Q and S.
The structure of TEV protease is similar to that of of the serine protease family.
Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds.
The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used.
The strain used is the autoinactivation-resistant mutant S219V.
The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1500-1:27,000Western Blot: 1:500, band of ~27kD is observedOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Specificity：Recognizes Tobacco Etch virus TEV Protease
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease
