Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes.
Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase- associated protein 1 (TP1).
Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death.
High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy, expression levels of TP1 do not correlate with malignancy.
At least two isoforms of TP1 are known to exist.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1-2ug/ml, a ~240kD band is expectedOptimal dilutions to be determined by the researcher.
Positive Control:Human kidney tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human TEP1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rb
Immunogen：Synthetic peptide corresponding to a region near the N-terminus of human telomerase associated protein 1 (TEP1). Species sequence homology: mouse and rat
