Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs).
The PDZ domain-containing A-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1mGluRs.
The C-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs)specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins.
Tamalin forms a protein complex with group 1mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins.
It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1mGluRs at synapses.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:20,000Western Blot: 1:500-1:3000Immunoprecipitation: 1ugOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Tamalin.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to mouse Tamalin at N-terminus.
