Swi6 is also known as chromatin-associated swi6 protein and repression of silent mating type loci protein.
This protein recognizes and binds to histone H3 tails that are methylated at Lys-9, leading to epigenetic repression.
Swi6 is also involved in the repression of the silent mating-type loci MAT2 and MAT3 and it may be resposible for the compaction of MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes.
Swi6 also interacts with the cohesin subunit psc3.
Swi6 has a nuclear localization and contains 2 chromo domains.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:30,000Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Saccharomyces pombe Swi6.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to aa314-328 of S.pombe Swi6.
