Apoptosis, or programmed cell death, is related to many diseases, such as cancer.
Apoptosis is triggered by a variety of stimuli, including members of the TNF family, and prevented by the inhibitor of apoptosis (IAP) proteins.
IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.
A novel IAP protein was recently identified and designated Survivin (also referred to as apoptosis inhibitor 4(API4) and TIAP).
Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity.
Survivin is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:1000ELISA: 1:10,000-1:40,000Western Blot: 1:200-1:2000, a ~17kD band is expectedImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified from monospecific antiserum by selective precipitation followed by ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Survivin.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region near the C-terminus of human Survivin.
