84-2136-27　SUMO (Small Ubiquitin-like Modifier, GAP modifying protein 1, GMP 1, PIC 1, Sentrin, Small ubiquitin related modifier 1) 500ug　168531

特徴

• Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.
• However, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome but rather seems to enhance stability or modulate their subcellular compartmentalization.
• Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.
• Examples of UBLs are SUMO, Rub1 (also called Nedd8), 8-12aa.
• Proteins of the second class, including parkin, RAD23 and DSK2, are designated ubiquitin- domain proteins (UDPs).
• These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.
• In contrast to UBLs, UDPs are not conjugated to other proteins.
• Once covalently attached to cellular targets, SUMO regulates protein:protein and protein: DNA interactions, as well as localization and stability of the target protein.
• Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.