STAT2 is a member of the STAT family of transcription factors.
Unlike other STATs, STAT2 is unique as it can only be activated by interferons (IFNs).
STAT2 is a critical component in mediating many IFN-stimulated biological activities including proliferation and viral responses.
Upon IFN treatment, STAT1 and STAT2 become tyrosine phosphorylated, assemble as heterodimers that bind IRF9 to form the ISGF3 complex.
This complex translocates to the nucleus, binds to promoters of IFN-stimulated genes and mediates gene transcription.
Consequently, mutations in STAT2 or loss of STAT2 expression leads to impairment in IFN signal transduction and gene activation.
IFN-a is an approved drug for the treatment of several forms of cancer.
Yet only a subset of patients who receive IFN-a therapy benefit from the treatment.
Given that STAT2 is activated by IFNs, it is important to define if the reduced or lack of tumor effects seen in cancer patients on IFN therapy is due to in defects in STAT2 function.
Our goal is to identify regions/motifs within the structural domains of STAT2 that not only are essential for the tyrosine phosphorylation of STAT2, but also regulate the tumor effects of IFN-a.
Collectively, the results of our studies will emphasize the physiological role of STAT2 in cancer.
From a clinical viewpoint, cancer patients who may benefit the most from receiving IFN-a therapy can be selected based on their STAT2 function.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:85,000Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human STAT2, phosphorylated at Y690. Species Crossreactivity: chimpanzee, macaque, Vervet monkey, rat, canine, porcine, equine, mouse and bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Eq Hu Mc Mk Mo Po Rt
Immunogen：Synthetic peptide corresponding to human STAT2 at N-terminus.
