Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation.
It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.
SPRY4 is widely expressed, with different isoforms.
The protein consists of 322aa, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence.
It is expressed predominantly in the cytoplasm.
Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000-1:12,000Western Blot: 1:100-1:1000Immunohistochemistry: 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Sprouty-4. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa306-322 from human Sprouty-4.
