TSmad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.
These activators exert diverse effects on a wide array of cellular processes.
The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.
Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g.
Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.
Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.
Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:75,000-1:300,000Western Blot: 1:2000-1:20,000Immunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human SMAD3 Phospho S423/phospho S425. Species Crossreactivity: Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, porcine, bovine and chicken.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ch Hu Mo Po Rt Xe Ze
Immunogen：Synthetic peptide corresponding to aa417-425 from human SMAD3.
