84-2136-10　SMAD2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18, JV18-1, MAD-related Protein 2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2) 100ug　168512

特徴

• Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.
• elegans gene Sma.
• SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
• This protein mediates the signal of the transforming growth factor (TGF)-b, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
• This protein is recruited to the TGF-b receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.
• In response to TGF-b signal, this protein is phosphorylated by the TGF-b receptors.
• The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.
• The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000Western Blot: 1:1000-1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.