SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors.
SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
In spinal chord development SLIT-2 may play a role in guiding commissural axons once they reach the floor plate by modulating the response to netrin.
In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex.
In vitro, only commissural axons that crossed the midline responded to SLIT-2.
In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm.
SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord.
Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined.
Multiple isoforms have been reported for this product.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1500-1:6000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human SLIT-2. Species Crossreactivity: chicken.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Hu
Immunogen：Synthetic peptide corresponding to aa484-500 from human SLIT-2.
