Sipa1(signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP).
It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases.
This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed.
Sipa1 is localized to the perinuclear region.
Two alternatively spliced variants encoding the same isoform have been characterized to date.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1.25-2.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Sipa1. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to mouse Sipa1 at N-terminal.
