84-2136-03　SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1) 500ug　168505

特徴

• SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response.
• SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion.
• Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy.
• This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1.
• SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus.
• The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung.
• Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-b, in cultured cells.
• SGK-1 is regulated by phosphorylation.
• Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at 422aa which in turn increases the phosphorylation of 256aa by PDPK1.
• The kinase is ubiquitinated by NEDD4L; which promotes proteasomal degradation.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:4000-1:16,000Western Blot: 1:500-1:3000Immunohistochemistry: 10-20ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.