S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 2kD.
In human brain tissue S-100 protein is mainly presented as two isoforms- bb homodimer (S-100b) and ab heterodimer (S-100a).
Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury.
Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream.
Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke.
Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues.
S-100 is an intracellular protein that weakly binds calcium.
It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium.
Distinct binding sites, with different affinities, exist for both ions on each monomer.
Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:3000Immunohistochemistry: 1:200-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
