84-2135-87　ROC1 (E3 Ubiquitin-protein Ligase RBX1, Protein ZYP, RING Finger Protein 75, RING-box Protein 1, Rbx1, Regulator of Cullins 1, RBX1, RNF75) 100ul　168486

特徴

• ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription.
• ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate.
• The RING-type zinc finger domain is essential for ubiquitin ligase activity.
• ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2.
• ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.