ROBO-1 (also called roundabout homolog 1 precursor and deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2.
The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex.
ROBO-1 may also be required for lung development.
ROBO-1 is a type I membrane protein.
ROBO-1 is a widely expressed protein with the exception of the kidney.
Defects in ROBO1 may be a cause of breast and lung cancer.
ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line.
Multiple splice variants have been identified for this protein.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:30,000-1:160,000Western Blot: 1:500-1:3000Immunohistochemistry: 2ug/ml-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human ROBO-1. Species Crossreactivity: mouse, rat and canine.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1632-1644 from human ROBO-1.
