RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, ring finger protein 1b, ring finger protein 2 and RNF2) is one of the PcG proteins.
The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation.
It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2).
Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior- posterior axis, as well as in cell proliferation in early development.
This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human RING1B. Species Crossreactivity: chimpanzee, orangutan, mouse, rat, canine, bovine, frog and chicken.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Ch Fr Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa189-201 from human RING1B.
