Sheep Red Blood Cell may be used in hemagglutination assays.
Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination.
Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid.
As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form.
Normally, a virus dilution (e.g.
2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g.
0.1% to 0.7% in steps of 0.2%) for approx.
30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs.
Then the lattice forming parts will be counted and the titer calculated.
The titer of a hemagglutination assay is determined by the last viable "lattice" structure found.
This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together.
SHEEP Red Blood Cell is used to sensitize erythrocytes and quantitate agglutination.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50mg
Host：rabbit
Source Antibody：sheep
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
