R-Phycoerythrin (240kD) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
Reaction with low molecular fragments is typically noted by Western Blot.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:200,000Western Blot: 1:2000-1:20,000Immunohistochemistry: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Specificity：Recognizes R-Phycoerythrin.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Highly purified R-Phycoerythrin from the seaweed gracilaria.
