PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, B-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells.
In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene.
Transcription of the PRDM1 gene is increased upon virus induction.
PRDM1/Blimp1 also interacts with PRMT5.
Two alternatively spliced transcript variants that encode different isoforms have been reported.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:65,000-1:80,000Western Blot: 1:1000Immunoprecipitation: 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human PRDM1 BLIMP1. Species Crossreactivity: mouse, rat and canine.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human, mouse, and rat PRDM1/BLIMP1 at C-terminus.
