84-2135-52　pRb2 p130 (130kD retinoblastoma associated protein, RBR2, Retinoblastoma like 2, Retinoblastoma like protein 2, Retinoblastoma Related Gene) 100ug　168443

特徴

• Spa310 is a 39aa long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene.
• Rb2 is a member of the retinoblastoma (Rb) gene family.
• Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors.
• Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation.
• Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition.
• Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle.
• Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:75,000-1:100,000Western Blot: 1:500-1:5000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.