POLK (also known as DNA polymerase-k, DINB protein and DINP) is involved in DNA repair.
POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.
Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts.
POLK lacks 3'-5' proofreading exonuclease activity.
POLK is a nuclear protein that binds to REV1L and PCNA.
POLK is detected throughout the nucleus and at replication foci.
Multiple alternative splice variants have been detected for this protein.
POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human POLK.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa817-830 from human POLK.
