84-2135-49　Pogz (pogo transposable element with ZNF domain, putative protein product of Nbla00003, SUHW5, suppressor of hairy wing homolog, zinc finger protein 280E) 100ug　168440

特徴

• Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family.
• Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors.
• Mouse Pogz has a human homolog with a base pair similarity of 90% and aa similarity of 93% for the full length protein.
• In humans at least 3 variants have been predicted and they are all similar at the N-terminus.
• A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells.
• In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000-1:400,000Western Blot: 1:2000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.