Mammalian PNK (also known as PNKP, polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and polynucleotide kinase) catalyzes the 5’ phosphorylation of nucleic acids and can have associated 3’ phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.
PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000-1:12000Western Blot: 1:300-1:1500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human PNK. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human PNK.
