Programmed cell death 4(Pdcd4) is a novel tumor supressor.
Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex.
Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun.
Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457.
The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:400,000Western Blot: 1:500-1:2000Immunohistochemistry: 2.125-2.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Pdcd4, phosphorylated at S457. Species Crossreactivity: mouse, rat and xenopus.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rb Rt Xe
Immunogen：Synthetic peptide corresponding to a region surrounding Ser457 from human Pdcd4.
