The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand.
PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression.
In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision.
PCNA is a useful marker for DNA synthesis and is highly conserved among most species.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:8000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human PCNA. Species Crossreactivity: monkey, canine, mouse, rat, bovine, chicken, fish and xenopus.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Ch Hu Mk Mo Rt Xe
Immunogen：Synthetic peptide corresponding to human PCNA.
