PC2 is the human homolog of the drosophila 'polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.
The human Pc homolog gene is more closely related to a xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1).
However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex.
The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation.
Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:400ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:4000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human PC2.
Isotype：IgG
Calc Applications Abbrev：E IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa95-107 from human PC2.
