RSK1 is a member of a family of 90kD ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3.
These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2.
Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation.
Active Rsk’s appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.
RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M tris chloride, 0.5M sodium chloride, pH 8.0, 0.01% sodium azide, 50% glycerol.
Specificity：Recognizes human p90 RSK1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human RSK1 at C-terminal.
