The protein p70 S6 kinase is critical for cell cycle progression and cell survival.
In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity.
p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome.
This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40S ribosomal subunit protein S6.
p70S6K is activated by diverse stimuli through multi-site phosphorylation at positions such as Thr-252 and Ser-434.
In Alzheimer's Disease, p70S6K activation is associated with PHF-tau (hyperphosphorylated tau) accumulation.
In non-neuronal cells, p70S6K has been shown to regulate actin polymerization.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Immunoprecipitation: 5ug/mgOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M tris chloride, 0.5M sodium chloride, pH 8.0, 0.01% sodium azide.
Specificity：Recognizes human P70 S6 Kinase.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human p70S6K at C-terminal.
